Fig. 5.

The effects of tryptophan and 5-HT on hypothalamic agouti-related proteins were studied by the GT1-7 cell line

Dose-response (A) and time course (B) of cell activity after in vitro administration of corticosterone; Dose-response (C) and time course (D) of cell viability after in vitro administration of 5-HT; (E) Incubation of GT1-7 cells with 5-HT (0.1, 1 μM) for 2 h before co-incubation with 10 μM corticosterone for 24 h prevented the cytotoxic effects of corticosterone. At the end of the treatment, cell viability was measured by the CCK8 assay. (F–K) In vitro administration of 5-HT prevented the mRNA expression of AgRP, OX1R, MC4R, LEPR, 5-HT_1B, and 5-HT_2C after corticosterone-induced cytotoxicity. Values are expressed as mean ± SEM. *P < 0.05, **P < 0.01. Significant differences between means were determined by t-test.