FIGURE 3.

Egg yolk vitamin D₃ metabolite concentrations from 78-wk Hy-Line Brown laying hens fed different concentrations of dietary vitamin D₃. (A) Cholecalciferol (vitamin D₃) (B) 25-hydroxycholecalciferol (25-OH-D₃). Blue squares denote standard NRC range vitamin D₃ concentrations (400 IU D₃/kg) in diet (n = 4), and red diamonds denote super-dose concentrations of vitamin D₃ (14,000 and 36,000 IU D₃/kg) in diet (n = 4). Samples were reported as means ± SEM; however, error bar values were narrow and overlapped by the marker for each sample. Samples with common letters were not significantly different from each other (general linear models, P < 0.0001). NRC, National Research Council; SEM, standard error of the mean.