FIG. 8.

Regions of class I molecules required for downregulation by K3 and K5. 221 cells stably expressing class I chimeric molecules were electroporated with GFP reporter, GFP-K3, or GFP-K5 vector. The level of MHC class I surface expression was assessed 48 h posttransfection by staining the cells with a PE-conjugated W6/32 antibody for class I (y axis) and gating the GFP-positive cell population (x axis) by flow cytometry. The numbers inside the boxes indicate the average mean values of MHC class I surface expression of the GFP-positive cell population. The data were reproduced in three independent experiments.