Fig. 3. Cell-type development of the tgrB1^– cheater and its victim.

We grew strains separately, mixed them at equal proportions, co-developed them, and imaged at the finger stage. a We mixed unlabeled wild-type AX4 cells with mutant tgrB1^– cells carrying the prestalk reporter [ecmA]:GFP and the prespore reporter [cotB]:RFP. b As a control, we mixed equal proportions of unlabeled AX4 cells with AX4 cells carrying the prestalk reporter [ecmA]:GFP and the prespore reporter [cotB]:RFP. We imaged the structures with DIC and with green and red fluorescence, and generated a merged image of the red and green channels, as indicated. Camera settings are included in Supplementary Data 1.