KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Rabbit polyclonal anti-ACTC1 | Thermo Fisher Scientific | Cat# PA5–21396; RRID: AB_11152296 |
| Rat monoclonal anti-CD45 (clone 30-F11) | Thermo Fisher Scientific | Cat# 14–0451-82; RRID: AB_467251 |
| Rabbit polyclonal anti-KRT5 | US Biological | Cat# C9097–37A2; RRID: AB_2134158 |
| Goat polyclonal anti-GATA6 | R and D Systems | Cat# AF1700; RRID: AB_2108901 |
| Rabbit monoclonal anti-PPARG | Thermo Fisher Scientific | Cat# MA5–14889; RRID: AB_10985650 |
| Goat polyclonal anti-PLP1 | Abcam | Cat# ab61682; RRID: AB_944751 |
| Goat polyclonal anti-PDGFRA | R and D Systems | Cat# AF1062; RRID: AB_2236897 |
| Chemicals, Peptides, and Recombinant proteins | ||
| DAPI | Invitrogen | Cat# D1306; RRID: AB_2629482 |
| TO-PRO-3 | Invitrogen | Cat# T3605 |
| WGA (CF405M conjugate) | Biotium | Cat# 29028 |
| WGA (AF488 conjugate) | Invitrogen | Cat# W11261 |
| WGA (AF647 conjugate) | Invitrogen | Cat# W32466 |
| HBSS | Sigma | Cat# H9394 |
| PBS | Sigma | Cat# D8537 |
| BSA | Sigma | Cat# A3311 |
| Dispase II | Gibco | Cat# 17105041 |
| DNase I | Roche | Cat# 10104159001 |
| Collagenase IA | Sigma | Cat# C2674 |
| Tamoxifen | Sigma | Cat# T5648 |
| Corn Oil | Sigma | Cat# C8267 |
| DIVA Decloaker | Biocare Medical | Cat# DV2004MX |
| Critical Commercial Assays | ||
| Chromium Single Cell 3’ kit v2 | 10X Genomics | Cat# PN-120237 |
| RNAscope Multiplex Fluorescent Reagent Kit v2 | ACDBio/Bio-Techne | Cat# 323100 |
| TSA Cy3, Cy5, TMR, Fluorescein Evaluation Kit | PerkinElmer | Cat# NEL760001KT |
| Deposited Data | ||
| Single-cell RNA-seq data | ArrayExpress | E-MTAB-11920 |
| Input files for analysis code and critical output files (cluster assignment, umap coordinates, h5ad files) | Zenodo | https://doi.org/10.5281/zenodo.7805311 |
| Online tool for visualization of single-cell data | Kasper Lab Website | http://kasperlab.org/tools/embryonicskin |
| Experimental Models: Organisms/Strains | ||
| Mouse: C57BL/6J | Charles River | JAX: 000664; RRID: IMSR JAX 000664 |
| Mouse: R26-tdTomato | Jackson Laboratory | JAX: 007908; RRID: IMRS JAX 007908 |
| Mouse: Gata6-EGFP-CreERT2 | Donati et al.177 | N/A |
| Mouse: Ebf2-EGFP-CreERT2 | Qian et al.178 | N/A |
| Oligonucleotides | ||
| See Table S4 for all oligonucleotides used in this study. | ||
| Software and Algorithms | ||
| Custom scripts and computational analysis workflow | Kasper Lab GitHub / Zenodo | https://github.com/kasperlab/Jacob_et_al_2023_Developmental_Cell (release v1.0; corresponds to https://doi.org/10.5281/zenodo.8152645) |
| Cell Ranger | 10X Genomics | https://github.com/10XGenomics/cellranger (release v2.0.0) |
| Seurat | Stuart et al.179 | https://github.com/satijalab/seurat (release v3.1.1) |
| Velocyto | La Manno et al.15 | https://github.com/velocyto-team/velocyto.py (release v0.17.17) |
| scVelo | Bergen et al.180 | https://github.com/theislab/scvelo (release v0.2.1) |
| Scanpy | Wolf et al.181 | https://github.com/theislab/scanpy (release v1.6.0) |
| Bbknn | Polánski et al.182 | https://github.com/Teichlab/bbknn (release v1.3.9) |
| CellRank | Lange et al.183 | https://github.com/theislab/cellrank (release v1.1.0 for epidermal cells & release v.1.5.1 for fibroblasts) |
| CellChat | Jin et al.69 | https://github.com/sqjin/CellChat (release v1.5.0) |
| Fiji | Schindelin et al.184 | https://fiji.sc |