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. 2024 Apr 3;16(4):evae067. doi: 10.1093/gbe/evae067

Table 1.

Comparison of assembly quality statistics and BUSCO search results among the three CCGP (left three) and three VGP (right three) genomes. BUSCO results for all genomes were obtained using the 8,338 universal single copy genes in birds found in the aves_odb10 database

Genome metrics Savannah Sparrow (Passerculus sandwichensis) Bell's Sparrow (Artemisiospiza belli) Song Sparrow (Melospiza melodia) Swamp sparrow (Melospiza georgiana) Nelson's sparrow (Ammospiza nelsoni) Saltmarsh sparrow (Ammospiza caudacuta)
Chromosomes NA NA NA 36 37 40
# contigs 676 1,539 823 276 292 645
Largest contig (bp) 32,137,824 35,931,659 59,497,540 29,976,604 50,792,433 43,812,934
Total length (bp) 1,152,258,190 1,401,798,777 1,356,272,071 1,160,782,308 1,180,370,373 1,239,216,328
GC (%) 43.1 43.46 44.45 43.24 43.01 43.5
N50 5,981,027 8,253,817 8,311,625 10,446,106 12,036,358 8,252,193
N75 2,762,208 1,578,947 3,378,466 3,855,203 4,591,448 3,265,797
L50 50 45 39 36 27 39
# scaffolds 337 1,339 501 40 77 282
Largest scaffold (bp) 124,432,526 99,814,828 153,992,920 155,044,423 155,447,619 157,152,855
Total length (bp) 1,152,292,115 1,401,818,823 1,356,304,709 1,162,015,399 1,185,463,352 1,241,209,685
GC (%) 43.1 43.46 44.45 43.24 43.01 43.5
N50 18,220,233 17,082,054 25,784,215 74,254,230 74,723,840 78,443,464
N75 6,722,078 2,980,250 6,297,809 23,937,375 21,551,278 22,481,186
L50 17 20 14 6 6 6
# N's per 100 kbp 2.94 1.43 2.38 106.12 429.62 160.60
BUSCO_results (%)
complete 95.2 95.6 95.5 94.9 94.8 95.4
complete and single copy 94.6 95.2 95.0 94.5 94.4 94.9
complete and duplicate 0.6 0.4 0.5 0.4 0.4 0.5
fragmented 2.1 1.5 1.6 1.8 1.9 1.8
missing 2.7 2.9 2.9 3.3 3.3 2.8