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(A) tRNA binds to the primer binding site, on the 5′ side of the HIV genomic RNA. (B) Reverse transcriptase extends the tRNA. (C) Extended tRNA is denatured from the 5′ side and anneal to complementary 3′ region of genomic RNA. (D) NCp7 disrupts both TAR hairpins, which is needed for reverse transcriptase to finish full strand reverse transcription. (E) Reverse transcriptase completes DNA transcription. (F) Polymerase finishes dsDNA.
