Extended Data Fig. 9. Persistent activation of astrocytic somata but not surrounding gliapil for strong stimulation of locus coeruleus, extension of Fig. 7n.
For these astrocytes, somata (blue) were activated shortly after gliapil (red) for weaker stimuli (10 s stimulation, 5 Hz, see Methods for details). For stronger stimulation (10 s stimulation, 20 Hz), somatic calcium signals were not simply a delayed version of gliapil signals but showed a persistent component. Such persistent activation was also observed without optogenetic stimulation during spontaneous behavior (Extended Data Fig. 7e,f).