Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2024 Mar 27;27(5):886–900. doi: 10.1038/s41593-024-01600-y

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Extended Data Fig. 4 — a, UMAP plot of the 38,360 mouse microglia isolated from 3 and 6 months App^NL-G-F and App^WT mice passing quality control, coloured by annotated cell states and after removal of CAMs, other myeloid, low quality and proliferating clusters. b, Phenotypic trajectory followed by the human microglia after exposure to amyloid-β in vivo, obtained by an unbiased pseudotime ordering with Monocle 3. c, Density plots displaying the average distribution of human microglia transplanted in 3 months old App^NL-G-F (n = 11) 6 months old App^NL-G-F (n = 9) and App^WT (n = 8) mice (density is normalized to sample size). d, Box plot showing proportion of cells across all identified clusters (eEach dot represents a single mouse, n = 8 for App^WT, n = 11 for App^NL-G-F 3 m, n = 9 for App^NL-G-F 6 m). e, Distribution of cells from different host mouse genetic backgrounds and ages (y-axis) across the DAM and HLA transcriptional trajectories colored by clusters shown in a (Each dot represents a single cell, n = 8 mice (8,974 cells) for App^WT, n = 11 mice (10,822 cells) for App^NL-G-F 3 m, n = 9 mice (12,197 cells) for App^NL-G-F 6 m). f, Phenotypic trajectory followed by the human microglia obtained by an unbiased pseudotime ordering with Monocle 3. Proportion of cells (y-axis) present in each cell state as in a (rows) at different stages of the binned pseudotime trajectory (x-axis) in 3 and 6 months old App^NL-G-F and App^WT mice (Each dot represents a single mouse, n = 8 for App^WT, n = 11 for App^NL-G-F 3 m, n = 9 for App^NL-G-F 6 m; *p < 0.05, ****p < 0.001). Box plots in d, e and f are limited by lower and upper quartiles and midline indicates median; whiskers show minimum to maximum value (d) or extend from the box to the smallest or largest value no further than 1.5*inter-quartile range (e and f). One-way ANOVA with Tukey’s multiple comparisons as post-hoc test, alpha = 0.05, significance was set as P-adjusted value < 0.05.