Fig. 6. Single-microglial nuclei from human postmortem brain.

a–e, Human snRNA-seq datasets from Gerrits et al.²⁰ (n = 176,136) (a), Sayed et al.³⁵ (n = 28,767) (b), Zhou et al.¹⁸ (n = 3,978) (c), Olah et al.³⁶ (n = 16,242) (d) and Sun et al.²¹ (n = 194,000) (e) re-analyzed to reproduce their original embeddings and annotated with our xenotransplanted microglial profiles as in Fig. 1b. f, Pairwise Pearson correlation between logFC of all differentially expressed genes (logFC cut-off set at 0.25, P < 0.05) of each microglial subtype and logFC of all differentially expressed genes (P < 0.05) of clusters from each human snRNA-seq study, with significance set at a P-adjusted value < 0.05 (*P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001). Only positive correlations are depicted here (r > 0). Additional correlations are shown in Extended Data Figs. 6 and 7. scRNA-seq, single cell RNA-sequencing.