Fig.2.

MLCK1 was expressed at different levels in different colon cancer cells, and high expression MLCK1 cell lines were less sensitive to 5-Fu. a–c results of western blot and RT-PCR show that expression levels of MLCK1 were higher in CACO2 and HT29 cells and lower in LOVO and SW480. d, e 24h IC 50 of 5-Fu for LOVO cells was 16.84ug/ml, for SW480 cells was 21.95ug/ml, which are much lower than that of HT29 cells(624.7ug/ml) and CACO2 cells(236.4ug/ml). f results of inhibition rate suggest that at a certain low concentration range, 5-Fu inhibited HT29 cells and CACO2 cells much more than SW480 cells and LOVO cells. g, h we divided the cells into two groups (CACO2 vs LOVO and HT29 vs SW480), and treated them with different concentrations of 5-Fu (10ug/ml, 100ug/ml vs control and 20ug/ml, 200ug/ml vs control), the flow apoptosis results suggested that HT29 and CACO2 cells have little apoptosis in the 5-Fu group which were not sensitive to 5-Fu. More cells apoptosis in SW480 and LOVO group, and apoptosis was significantly higher, statistically significant difference (p < 0.05). i, j flat dish monoclonal experiment, divided into two groups, with different concentrations of 5-Fu(control/10/20/100ug/ml), and the results showed that at the same concentration of 5-Fu, SW480 cells and LOVO cells had fewer clones than HT29 cells and CACO2 cells