Fig.4.

Results of stable transcendental overexpression of MLCK1 in SW480 cell. a result of western-blot of control/transient/stable transcendental overexpression of MLCK1 in SW480 cell line. b results of cell inhibition rate at different 5-Fu concentrations suggested the drug inhibited SW480 MYLK cell line at a much lower rate than SW480 cell line. c, d at different concentrations of 5-Fu administration (20ug/ml; 200ug/ml) with the control group suggest that after stable transfection of overexpressed MLCK1 apoptosis of SW480 cells was reduced, p < 0.05, statistically significant difference. e, f we performed cell sequencing of the stably transfected MLCK1 overexpressing cell line SW480 MYLK, and the results suggested that the enhanced drug resistance may be achieved by MLCK1 through TNFR2/NF-κB pathway interactions. g autophagy-related pathway proteins changed after stable transfection with MLCK1 overexpression in SW480 MYLK, i.e., LC3B II/Beclin-1 expression decreased and P62 expression increased, indicating that the level of death autophagy in intestinal cancer cells decreased