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. 2024 Apr 29;14:1368622. doi: 10.3389/fcimb.2024.1368622

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Copyright © 2024 Carrera Páez, Olivier, Gambino, Poklepovich, Aguilar, Quiroga and Centrón

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Phenotypic characterization of EV from native and evolved XDR-E. coli M19736. Vesicles were purified from broth cultures by ultracentrifugation and filtered through a 0.22-μm filter. DLS results are presented as the mean of three independent measurements ± SD. DLS measurement shows an average size of 193.2 ± 1.8 nm for the native E. coli M19736 (A) and 174.7 ± 0.52 nm for the evolved XDR-E. coli M19736 (C). TEM results show in both strains EV with a double membrane, spherical in shape and heterogeneous in size (B, D).