| Antibody | Concentration (Immunofluorescence) | Source and identifier |
| Rabbit anti-Atoh1 | 1:500 | Proteintech 21,215-1-AP |
| Rabbit anti-ꞵ-galactosidase | 1:500 | Invitrogen A11132 |
| Rat anti-BrdU | 1:500 | AbCam AB6326 |
| Rabbit anti-Lmx1 | 1:2000 | Millipore AB10533 |
| Goat anti-Msx1 | 1:50 | Rndsystems AF5045 |
| Alexa Fluor 594 Chicken anti-Goat IgG (H + L) | 1:1000 | Invitrogen A-21468 |
| Alexa Fluor 488 Chicken anti-Rabbit IgG (H + L) | 1:1000 | Invitrogen A-21441 |
| Alexa Fluor 488 Goat anti-Rat IgG (H + L) | 1:1000 | Jackson ImmunoResearch AB_2338351 |
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