FIG. 1.

FV-specific IFN-γ production by spleen cells from FV-infected H-2^b/b mice. Spleens were removed from either uninfected H-2^b/b mice or H-2^b/b mice infected with 1,500 SFFU at 10 days postinfection. Spleen cells from these mice were depleted of red blood cells and then cultured with irradiated syngeneic spleen cells from naive mice as APCs and either 5 μg of purified FV per ml or no antigen. After 72 h, supernatants were harvested and analyzed for IFN-γ using an IFN-γ-specific sandwich ELISA. Rat anti-mouse IFN-γ MAb R4-6A2 (Pharmingen, San Diego, Calif.) was used as the coating antibody, and biotinylated XMG1.2 (Pharmingen) was used as the detection antibody. Data are presented as the means plus the standard errors of the means for three mice per group.