FIG. 6.

FV-specific IFN-γ production by T-cell subsets. CD4⁺ T cells and CD8⁺ T cells from spleens of FV-infected H-2^b/b and H-2^bm14/b mice were purified by positive selection using the MidiMACS separation system (Miltenyi Biotech, Bergisch Gladbach, Germany) and analyzed for the number of IFN-γ-producing cells by ELISPOT analysis as described for Fig. 5. Cell purity of each population was measured using fluorescein isothiocyanate-labeled anti-CD3 (145-2C11), anti-CD4 (GK1.5), anti-CD8 (169.4), or Thy1.2 (all purchased from Pharmingen). Data are presented as the number of IFN-γ-producing cells per 10⁶ cells of each specific cell type for each mouse strain. In experiment 1, data are the means plus the standard errors of the means of six points for each mouse strain, whereas in experiment 2, data are the means plus the standard errors of the means of two points per strain.