Figure 8.
EZ Clear preserves fluorescence signal from endogenous fluorescent reporter transgenes
(A) Whole-mount lightsheet image, dorsal view, of the brain from a Tg(Thy1::GFP) (MGI# 3766828)11 adult mouse. Neurons should be identifiable and background signal should be minimal upon successful clearing.
(B‒D) Magnified images show individually labeled neurons through the brain.
(E) GFP positive cells are pseudocolored according to their depth in the imaging plane. This brain was imaged by AxL Cleared Tissue LightSheet (AxL CTLS) from Intelligent Imaging Innovations.
Scale bar in A = 1500 μm; B = 100 μm; C = 100 μm; D = 300 μm; E = 50 μm.