Table 2.
Detailed lightsheet imaging acquisition parameters utilized for individual organs from young and aged adult mice
| Organs | Laser (nm) | Emission Filter (nm) |
Zoom | Laser power | Exposure time (ms) | Lightsheet Thickness (μm) |
Overlap (%) | Raw file Size (Gb) |
Recon File size (Gb) |
|---|---|---|---|---|---|---|---|---|---|
| 2 month old mice perfused with 649-nm tomato lectin | |||||||||
| Eye | 638 | 660 | 0.7 | 14% | 119.85 | 7 | 15 | 28.3 | 10.4 |
| Kidney | 638 | 660 | 0.7 | 14% | 119.85 | 7 | 15 | 66.6 | 39.6 |
| Heart | 638 | 660 | 0.7 | 10% | 119.85 | 7 | 15 | 123 | 53.5 |
| Testis | 638 | 660 | 0.7 | 18% | 149.81 | 7 | 15 | 43.3 | 18.1 |
| #Brain | 640 | 655 | 3.25 | 200 mW | 300 | 10 | 15 | 201.6 | 54.8 |
| 5 month old | |||||||||
| Eye | 638 | 660 | 0.7 | 18 | 249.67 | 5.25 | 15 | 20.6 | 7.4 |
| Kidney | 638 | 660 | 0.7 | 14 | 119.85 | 7 | 15 | 83.6 | 39.9 |
| Heart | 638 | 660 | 0.7 | 10 | 119.85 | 7 | 15 | 95.9 | 39.9 |
| Spleen | 638 | 660 | 0.7 | 14 | 119.85 | 7 | 15 | 102 | 45.7 |
| Ovary | 638 | 660 | 1.0 | 18 | 249.67 | 6 | 15 | 33.6 | 6.0 |
| Brain | 488 | 498 | 0.7 | 7.5 | 99.87 | 4.88 | 10 | 371 | 124 |
∗For all images, the lens magnification is 5X.
∗For all images, “Online Dual Side Fusion” with “Mean”, “Pivot Scan” and “Bi-directional” were checked.
#Imaged by AxL Cleared Tissue LightSheet (AxL CTLS) from Intelligent Imaging Innovations. The rest of the samples were imaged by Zeiss Z7.