4% paraformaldehyde (PFA) (500 mL)
| Reagent | Final concentration | Amount |
|---|---|---|
| ddH2O | N/A | 450 mL |
| PFA | 4% | 20 g |
| 10 N NaOH | N/A | A few drops to dissolve PFA powder |
| HCl (36.5%–38%) | N/A | A few drops needed to adjust pH to 7.2 |
| 10X PBS | 1X PBS | 50 mL |
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