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Incorporation of gp41 into virions. (A to E) Virions were pelleted, lysed, and analyzed by immunoblotting with the Chessie 8 anti-gp41 monoclonal antibody, as previously described (18). Arrows indicate gp41 or gp160. −Env is a negative control. WT, wild-type sequence from the HXB2 clone of HIV-1. ∗, nonspecific bands; ∗∗, probable gp41 dimer. Gels are representative of at least three independent experiments.
