Fig. 2.

Expression pattern of Npas4 in mouse olfactory bulbs for the postnatal developing period. (A~D) Representative images of the P0, P14, P28, and P56 olfactory bulbs. Cells are co-stained with the Npas4, Tbr2, and DAPI and imaged using confocal microscopy. Blue represents nuclear DNA stained with DAPI, red represents Tbr2, and green represents Npas4 (primarily expressed in the nucleus). Each layer of the olfactory bulb glomerular layer (GL), external plexiform layer (EPL), mitral cell layer (MCL), and granule cell layer (GCL) is indicated in each image. (E) A rectangular region of interest (ROI) of the same size was randomly placed in the image data of each layer, and the ratio of Npas4 expressing cells to the total cells and the ratio of Npas4 expressing cells to the Tbr2+ cells inside the ROI was counted and the intensity of Npas4 expressing cells was measured. The counts for Npas4+ cells and total cells are all listed in Table 4. The counts for Npas4+ cells and Tbr2+ cells are all listed in Table 5. Scale bar: 100 μm.