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. Author manuscript; available in PMC: 2024 May 13.
Published in final edited form as: Mol Cell. 2023 Sep 7;83(17):3095–3107.e9. doi: 10.1016/j.molcel.2023.08.006

Figure 3. rRNA transcription controls nucleolar shape.

Figure 3.

(A) Inhibition of RNA transcription by ActD for 4 h results in morphological changes of the nucleolus. Scale bar, 5 μm.

(B) Within a simple shape model (STAR Methods), FC location is sufficient to describe nucleolar shape in HEK, MV, and U2OS (left, middle, right). Scale bar, 1 μm.

(C) RNA (EU; white) is transcribed at the interface of FC (RPA16; cyan) and DFC (fibrillarin; yellow) (Scale bar, 5 μm.

(D) The RDF of nucleolar phases reflects their concentric layering (N = 131 nucleoli).

(E) rRNA is primarily located in the DFC 30 min after addition of EU to the media (N = 198 nucleoli); the DFC RDF fit is from (D). Shaded error region on RDFs are mean prediction bands to spline fits (supplemental information).