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. Author manuscript; available in PMC: 2024 May 13.
Published in final edited form as: Nat Methods. 2024 Jan 17;21(2):228–235. doi: 10.1038/s41592-023-02157-7

Extended Data Fig. 2 ∣. Coefficient of variation for technical replicates is negatively correlated with number of cells sequenced.

Extended Data Fig. 2 ∣

Scatter plot of the average number of cells for that species versus the coefficient of variation (CV) of gene prevalence for two technical replicates for every gene-species pair. CV is calculated as the standard deviation divided by the mean. The negative Spearman correlation (Rho) suggests that a moderate fraction of the variance found between technical replicates can be attributed to stochasticity due to small numbers of cells. P-value is based off comparison to a two-sided t-test null distribution.