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. Author manuscript; available in PMC: 2024 May 13.
Published in final edited form as: Nat Microbiol. 2023 May 4;8(6):1108–1122. doi: 10.1038/s41564-023-01380-4

Figure 2. Virological characterization of myotis bat morbillivirus (MBaMV).

Figure 2.

a, Syncytia formation in Vero-bCD150 cells induced by MBaMV 3 days post-infection (dpi). Cells formed syncytia involving > 100 nuclei upon infection (bright field), which is clearly outlined by virus expressed GFP (right). Scale bar equals 500 micrometers. This experiment was performed 3 times and representative images are depicted. b, MBaMV plaque formation in Vero-bCD150 cells. Cells were infected by 10-fold serially diluted virus stock, incubated with methylcellulose containing-DMEM and stained with crystal violet and neutral red 7 dpi. Diameter of well is 22 mm. One well is magnified to show the plaque morphology in detail. c, shows transmission electron microscopy (TEM) images of MBaMV virion on the surface of Vero-bCD150 cells at 3 dpi. Numerous enveloped virions are budding from or are associated with the plasma membrane (left). Filamentous particles can also be seen (asterisks). Magnified image (right) shows virion and ribonucleoprotein complex (RNP). These images are from one independent experiment.