Table 1.
Related animal and cell research with ADSCs
| Year | Authors | Experimental models | Stem cell types | Injection methods | Stem cell source | Result |
|---|---|---|---|---|---|---|
| 2023 | Li et al. [54] | Mouse and hypertrophic scar fibroblasts (HSF) | Adipose derived stem cells (ADSCs) -culture medium (CM) | Subcutaneous injection | Subcutaneous adipose tissue | A novel peptide derived from ADSC-CM attenuated hypertrophic scar fibrosis in vitro and in vivo. |
| 2022 | Li et al. [111] | Nude mouse and HSFs | ADSCs | Injected in the hypertrophic scar implant | Bilateral thighs and buttocks | ADSCs efficiently cured hypertrophic scars by promoting the apoptosis of HSFs and by inhibiting their proliferation and migration. |
| 2022 | Zhou et al. [126] | Keloid fibroblasts (KFs) | ADSCs | — | Human subcutaneous adipose tissues | ADSCs strongly suppressed KFs’ proliferative and invasive behavior, negatively regulated KF apoptosis. |
| 2022 | Xie et al. [106] | Rabbit ear and HSFs | ADSCs | Intradermal injection | Groin adipose tissue | ADSCs demonstrated the ability to prevent hypertrophic scar (HTS) formation via inhibiting the proliferation and migration of the synthesis of extracellular matrix of HSFs. |
| 2022 | Li et al. [127] | KFs | ADSC- Exosomes (Exo) | — | Human subcutaneous adipose tissues | ADSC-Exos inhibited ECM deposition in keloids, which may have been mediated by inhibition of the TGF-β2/Smad3 and Notch-1 signaling pathways. |
| 2021 | Zhang et al. [121] | Rabbit ear and human KFs | ADSC-CM | — | Inguinal fat tissues of rabbits | ADSC-CM can downregulate the expression of α-SMA due to its anti-fibrosis effect and promote the rearrangement of collagen fibres, which is integral to scar precaution. |
| 2021 | Xie et al. [79] | HSFs and KFs | ADSCs | — | Human subcutaneous adipose tissues | ADSCs can affect the biological behavior of HSFs and KFs in vitro by regulating the TGF-β1/Smad pathway. |
| 2021 | Wu et al. [124] | KFs | ADSC-Exo | — | Liposuction | ADSCs-EXO may inhibit the expression of the TGF-β1/Smad pathway, and thereby inhibit the proliferation, migration, and collagen synthesis of KFs. |
| 2021 | An et al. [50] | Nude mouse | ADSC-secretome | Applied to wound | Human subcutaneous adipose tissues | ADSC secretome can be effectively produced via maturation process, and safely utilized to restore damaged tissue architecture in clinical cases. |
| 2021 | Li et al. [118] | Dorsal skin of mouse and HSFs | ADSC-Exo | Subcutaneous injection | Human subcutaneous adipose tissues | ADSC-Exo attenuated the deposition of collagen, the trans-differentiation of fibroblasts-to-myofibroblasts, and the formation of hypertrophic scar by in vitro and in vivo experiments. |
| 2021 | Xu et al. [72] | Dorsal skin of mouse | ADSCs | Intravenous injection | Dorsocervical subcutaneous region | During wound healing, ADSCs may have antifibrotic potential by altering macrophage polarization. |
| 2021 | Yuan et al. [51] | Dorsal skin of mouse and HSFs | ADSCs-Exo | Subcutaneous injection | ADSC | ADSC exosome therapy can decrease scar formation by inhibiting the TGF-β2/Smad3 signaling pathway. |
| 2021 | Zhou et al. [52] | Dorsal skin of mouse | ADSC-Exo | Subcutaneous injection | Human subcutaneous adipose tissues | ADSC can effectively promote skin wound healing while inhibiting scar formation at the wound. |
| 2020 | Lu et al. [56] | Dorsal skin of mouse, HSFs, and human microvascular endothelial cells (HMECs) | ADSC-extracellular vesicles (EV) | Subcutaneous injection | Abdomen subcutaneous adipose tissues | The miR-486‐5p secreted from ADSC‐EVs possesses the capacity to promote HSFs migration and proliferation as well as HMECs angiogenesis. |
| 2020 | Zhu et al. [57] | Rabbit ear | ADSC-EVs | Injected at the edge and base of wound | Abdomen subcutaneous adipose tissues | A local injection of hASC-EVs efficiently prevented hypertrophic scar formation by suppressing myofibroblast aggregation and collagen deposition. |
| 2019 | Luo et al. [135] | Dorsal skin of mouse | ADSCs | Injected into the surface of the wound | Inguinal subcutaneous adipose tissues | ADSC partially modulates microskin function and enhances wound healing by promoting angiogenesis in a full-thickness skin defect mouse model. |
| 2018 | Wang et al. [129] | KFs | ADSC-CM | — | Human subcutaneous adipose tissues | ADSC-CM downregulated the extracellular matrix-related gene expression of PAI-1, TIMP-1, and COL1. |
| 2018 | Chu et al. [113] | Scar fibroblasts | ADSCs | — | Inguinal subcutaneous adipose tissues | ADSCs can inhibit the mRNA and protein expressions of α-SMA and promote the mRNA and protein expressions of DCN in in vitro culture system. |
| 2017 | Foubert et al. [101] | Porcine | ADSCs | Nasal spray device | Inguinal fat pad | Autologous ADSC administration reduced the HTS development following deep-partial cutaneous injury. |
| 2015 | Zhang et al. [130] | Rabbit ear | ADSCs and ADSC-CM | Intralesional injection | Groin fat pads | ADSCs reduced the formation of rabbit ear hypertrophic scars by decreasing the α-SMA and collagen type Ι gene expression and ameliorating collagen deposition. |
HSF, hypertrophic scar fibroblasts; ADSCs, adipose derived stem cells; ADSC-culture medium, ADSC-CM; KFs, keloid fibroblasts; HTS, hypertrophic scar; ADSC-Exo, ADSC-Exosomes; HMECs, human microvascular endothelial cells