Table 2.

Levels (pmol/mg of protein) of specific ceramide, monohexosylceramide, sphingomyelin and ceramide-1-phosphate species among the different groups in 661W cells.

Ceramide (pmol/mg)	Control	L-Cs only	H2O2 only	L-Cs + H2O2
C14:0	0.63 ± 0.09	0.81 ± 0.14	0.57 ± 0.11	0.59 ± 0.14
C16:0	25.36 ± 2.77	25.50 ± 4.25	31.05 ± 9.33	21.51 ± 3.15
C16 DH	1.67 ± 0.19	1.66 ± 0.18	15.25 ± 4.31b	4.93 ± 0.65 f
C18:1	13.67 ± 4.08	16.00 ± 3.67	25.21 ± 8.75	19.54 ± 4.82
C18:0	4.76 ± 1.05	4.67 ± 0.8	5.40 ± 1.79	4.89 ± 0.7
C20:0	4.31 ± 0.38	3.68 ± 0.43	4.56 ± 1.52	2.66 ± 0.23
C22:0	27.10 ± 2.15	26.49 ± 2.10	22.27 ± 6.9	14.42 ± 1.32
C24:1	53.50 ± 5.01	47.69 ± 4.78	39.12 ± 9.81	31.22 ± 3.73
C24:0	56.43 ± 6.13	51.59 ± 5.64	29.35 ± 8.14 c	21.54 ± 2.51
C26:1	21.60 ± 2.5	18.00 ± 2.22	10.60 ± 3.09 b	8.01 ± 0.65
C26:0	0.93 ± 0.12	0.70 ± 0.15	0.48 ± 0.11 c	0.26 ± 0.02 f
Monohexosylceramide (pmol/mg)	Control	L-Cs only	H2O2 only	L-Cs + H 2 O 2
C14:0	1.20 ± 0.15	1.73 ± 0.21	1.15 ± 0.15	1.05 ± 0.21
C16:0	216.71 ± 44.32	228.93 ± 31.8	125.61 ± 44.32	101.75 ± 31.8
C16 DH	2.45 ± 1.38	0.42 ± 0.09	0.72 ± 1.38	0.57 ± 0.09
C18:1	32.53 ± 9.07	39.00 ± 5.92	25.98 ± 9.07	21.06 ± 5.92
C18:0	19.58 ± 3.02	23.85 ± 3.8	16.81 ± 3.02	13.06 ± 3.8 f
C20:0	11.52 ± 1.36	12.98 ± 1.55	11.68 ± 1.36	8.04 ± 1.55 f
C22:0	119.15 ± 5.94	130.37 ± 13.14	107.95 ± 5.94	65.36 ± 13.14 e
C24:1	133.63 ± 7.84	146.68 ± 14.74	121.48 ± 7.84	90.82 ± 14.74
C24:0	272.46 ± 17.6	359.24 ± 34.20	245.00 ± 17.6	179.88 ± 34.2 f
C26:1	22.06 ± 1.72	23.80 ± 2.18	19.78 ± 1.72	14.79 ± 2.18 f
C26:0	3.60 ± 0.26	3.57 ± 0.33	2.74 ± 0.26	1.76 ± 0.33 f
Sphingomyelin (pmol/mg)	Control	L-Cs only	H2O2 only	L-Cs + H 2 O 2
C14:0	29.53 ± 2.49	24.63 ± 1.88	13.24 ± 2.01 a	12.85 ± 0.79
C16:0	275.04 ± 34.24	298.71 ± 27.41	188.19 ± 148.51	180.11 ± 26.18
C16 DH	24.75 ± 4.55	24.18 ± 4.20	27.90 ± 23.81	24.88 ± 3.92
C18:1	6.82 ± 1.71	7.074 ± 1.4	2.68 ± 3.27	3.07 ± 0.83
C18:0	59.06 ± 7.60	49.77 ± 8.18	34.09 ± 24.68	27.29 ± 4.51
C20:0	17.13 ± 0.99	15.23 ± 1.12	9.36 ± 5.7	9.19 ± 0.5
C22:0	67.63 ± 3.88	61.08 ± 4.96	36.41 ± 23.49	31.59 ± 2.31
C24:1	178.86 ± 11.73	170.84 ± 8.93	107.17 ± 64.13	95.65 ± 5.53
C24:0	90.12 ± 7.77	87.99 ± 6.42	48.73 ± 33.27	40.87 ± 3.23
C26:1	2.77 ± 0.3	2.83 ± 0.19	1.56 ± 0.95	1.30 ± 0.09
C26:0	0.81 ± 0.09	0.79 ± 0.07	0.43 ± 0.25	0.35 ± 0.03
Ceramide-1-phosphate (pmol/mg)	Control	L-Cs only	H2O2 only	L-Cs + H2O2
C14:0	4.50 ± 0.35	4.22 ± 0.26	4.52 ± 0.97 c	4.76 ± 0.24
C16:0	0.50 ± 0.12	0.33 ± 0.07	0.68 ± 0.59	0.46 ± 0.08

Footnote: Semiquantitative levels (pmol/mg of protein) of specific ceramide, monohexosylceramide, sphingomyelin and ceramide-1-phosphate species among the different groups in 661W cells (n = 6/group). Control: cells having no treatment with L-Cs or H₂O₂; L-Cs only: treated with 10 μM L-Cs for 3 h; H₂O₂ only: treated with 300 μM of H₂O₂ for 3 h; L-Cs + H₂O₂: cotreated with 10 μM L-Cs and 300 μM H₂O₂ for 3 h. Values are represented as mean ± SEM; t-test;

^aP < 0.001 between H₂O₂ only treated group and control;

^bP < 0.01 between H₂O₂ only treated group and control;

^cP < 0.05 between H₂O₂ only treated group and control;

^eP < 0.01 between L-Cs + H₂O₂ treated group and H₂O₂ only treated group;

^fP < 0.05 between L-Cs + H₂O₂ treated group and H₂O₂ only treated group;