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Phylogenetic tree of firefly luciferases and related ACSs. Known luciferases are shown in green letters and novel luciferase-like proteins are shown in red letters. A green box indicates Clade-I, and a red box indicates Clade-II. Subclade-IA, -IB, -IC, and -ID are indicated by yellow, blue, orange, and green bars, respectively. Gene expression levels (TPM) in lantern are shown in the logarithm. Black circles show the presence of the ‘Firefly-Luc-like’ motif (Motif) and PTS1.
