Figure 4.

WGCNA analysis, RT-qPCR and WB results. (A) Clustering dendrogram of DEGs revealing 5 module eigengenes (MEs), including MEturquoise, MEbrown, MEblue, MEyellow and MEgrey. (B) Heatmap plot of gene network displaying topological overlap, with light colors denoting low overlap and progressively darker colors higher overlap. Blocks of darker colors along the diagonal are the identified modules. (C) Module-trait relationship by Pearson correlation analysis. Each row corresponds to a ME and each column to a trait. Correlation coefficients (r value, scaled by colors) and corresponding P values are displayed in each rectangle. Positive correlation (Red); Negative correlation (Green); No correlation (White). (D) The qPCR results of Mrc1, Gpnmb, Aqp9, Trem1, Msr1, Slc26a4, Pde3a and Bmp6. Abbreviations: RNE, RNE = 2^−∆∆Ct. (E) The WB results of Mrc1, Gpnmb, Aqp9, Trem1, Msr1, Slc26a4, Pde3a and Bmp6 (n=4). *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001.

Abbreviation: ns, not significant.