Figure 3.

Effect of antiretroviral and selinexor treatment on nuclear export of HMGB1 in the PC9 cell line. (A) PC9 cells were treated with TNF-A (100ng/mL) to induce nuclear export of HMGB1, using different time intervals. Proteins from the nucleus and cytoplasm were separated and HMGB1 was detected by Western blotting. (B) PC9 cells were pre-treated with selinexor or antiretroviral drugs for 30 minutes and cells were then exposed to TNF-A (100 ng/mL) for 8 hours. Proteins from the nucleus and cytoplasm were separated and HMGB1 was detected by Western blotting. Data were analyzed using unpaired t-test comparisons.*P <0.05, **P <0.01, comparison between two groups marked with horizontal lines.