. 2024 May 1;22(5):e3002592. doi: 10.1371/journal.pbio.3002592

Table 3. Comprehensive validation comparing simulations against known experimental observations.

In the 47 total simulations in the table, 43 are consistent and, 4 are inconsistent (marked with bold, underlined font), with accuracy 43/47 = 91%.

Row #	Signal	Simulation setting	Experimental outcome	Ref	Simulated percentage of node
Consistent cases
1.	High CO₂	MPKs = OFF	No closure	[14,18]	Closure = 0%
2.	High CO₂	MPK activity = OFF	Closure	[15,16]	Closure = 100%
3.	High CO₂	MPK activity = ON	Closure	[16]	Closure = 100%
4.	High CO₂	MPK activity = OFF, MPKs-HT1complex = OFF	No or reduced closure	[16]	Closure = 0%
5.	High CO₂	HT1 = OFF	Closure	[11,13]	Closure = 100%
6.	High CO₂	HT1 = ON	No closure	[12,13]	Closure = 0%
7.	High CO₂	RHC1 = OFF, HT1 = OFF	SLAC1 CO₂ activation	[17]	SLAC1 CO₂ = 100%
8.	High CO₂	HT1 = OFF, OST1 minimum function = OFF	No or reduced Closure	[17]	Closure = 0%
9.	High CO₂	CBC1/2 OFF	Closure	[15]	Closure = 100%
10.	High CO₂	CAs = OFF	Reduced closure	[8]	Closure = 0%
11.	High CO₂	CAs = OFF, HT1 = OFF	Closure	[8]	Closure = 100%
12.	High CO₂	GHR1 = OFF	Reduced closure	[13,58]	Closure = 41.5%
13.	High CO₂	HCO₃^- = OFF, high CO₂ = ON	SLAC1 OFF	[9]	SLAC1 generic = 0%, SLAC1 CO₂ = 0%
14.	High CO₂	Ca²⁺_cyt = OFF	No closure	[19–21]	Closure = 0%
15.	High CO₂	ABI1 = ON	Reduced closure	[5,78]	Closure = 44.0%
16.	High CO₂	ABI2 = ON	No closure	[5,78]	Closure = 39.9%
17.	High CO₂	Default (high CO₂ = ON)	Ca²⁺_cyt > 0	[19]	Ca²⁺_cyt = 53.6% (oscillating)
18.	High CO₂	ROS = OFF	Reduced closure	[22]	Closure = 0%
19.	High CO₂	RbohD/F = OFF	Reduced closure	[22,79]	Closure = 0%
20.	High CO₂	OST1 minimum function = OFF	No closure	[9]	Closure = 0%
21.	High CO₂	Aquaporins = OFF	No closure	[7]	Closure = 0%
22.	High CO₂	CO₂ porins = OFF	No closure	[7]	Closure = 0%
23.	High CO₂	Default (high CO₂ = ON)	Anion efflux ON	[32]	AnionEM = 100%
24.	High CO₂	Default (high CO₂ = ON)	K⁺ efflux is ON	[32]	K⁺ efflux = 100%
25.	High CO₂	QUAC1 = OFF	Slower closure	[67]	Closure reaches 100% more slowly than WT
26.	High CO₂	CPKs = OFF (CPK 4,6,21,23)	Closure	[80]	Closure = 100%
27.	High CO₂	Default (high CO₂ = ON)	ROS is produced	[22,23]	ROS = 100%
28.	High CO₂	GHR1 = OFF, HT1 = OFF	No or reduced Closure	[13]	Closure = 43.9%
29.	High CO₂	SLAC1 CO₂ = OFF, SLAC1 generic = OFF	No closure	[81,82]	Closure = 43.9%
30.	High CO₂	SLAC1 CO₂ = OFF, SLAC1 generic = OFF, QUAC1 = OFF	No closure	[82]	Closure = 0%
31.	High CO₂	Default (high CO₂ = ON)	H⁺-ATPase deactivation	[83]	H⁺-ATPase = 0%
32.	Ca²⁺	MPKs = OFF, high CO₂ = OFF, CaIM = ON	Reduced AnionEM	[37]	AnionEM = 49.2%
33.	Ca²⁺	RHC1 = OFF, CaIM = ON, high CO₂ = OFF	Closure	[17]	Closure = 100%
34.	Ca²⁺	CaIM = ON, high CO₂ = OFF	Closure	[21]	Closure = 100%
35.	Ca²⁺	CPKs = OFF, CaIM = ON, high CO₂ = OFF	Reduced closure	[21]	Closure = 41.1%
36.	ROS	GHR1 = OFF, ROS = ON, high CO₂ = OFF	No closure	[48]	Closure = 41.2%
37.	ROS	ROS = ON, high CO₂ = OFF	Closure	[62,79]	Closure = 100%
38.	ROS	ROS = ON, RbohD/F = OFF	Closure	[79]	Closure = 100%
39.	NO	NO = ON, high CO₂ = OFF	Closure	[62,84]	Closure = 100%
40.	8-nitro-cGMP	8-nitro-cGMP = ON, high CO₂ = OFF	Closure	[38]	Closure = 100%
41.	cADPR	cADPR = ON, high CO₂ = OFF	Closure	[85]	Closure = 100%
42.	HCO₃	HCO₃^- = ON, high CO₂ = OFF	SLAC1 activation	[8]	SLAC1 CO₂ = 100%
43.	HCO₃	MPKs = OFF, high CO₂ = OFF, HCO₃^- = ON	Impaired SLAC1	[13,14,18]	SLAC1 generic = 0%, SLAC1 CO₂ = 0%
Inconsistent cases
44.	High CO₂	CBC1/2 = OFF, HT1 = ON	Closure	[15]	Closure = 0%
42.	High CO₂	NO = OFF	Reduced closure	[24]	Closure = 100%
46.	High CO₂	R256 residue mutation interpreted as SLAC1 CO₂ = OFF	Reduced closure	[60]	Closure reaches 100% more slowly than WT
47.	Ca²⁺	GHR1 = OFF, CaIM = ON, high CO₂ = OFF	Closure	[48]	Closure = 40.3%