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. Author manuscript; available in PMC: 2024 May 13.
Published in final edited form as: NPJ Metab Health Dis. 2024 Feb 21;2:2. doi: 10.1038/s44324-024-00004-7

Fig. 3 |. Diminished Spargel expression in the heads of dfmr1 mutants is restored by normalization of IS.

Fig. 3 |

A Western analysis of Spargel expression in extracts from iso31Bw- and dfmr1 mutant fly heads. An antibody to PGC-1α was used to detect Spargel expression (top). β-Tubulin was used as a loading control (bottom). See Supplementary Fig. 3 for full blots. B Quantification of the intensity of Spargel relative to β-Tubulin. An unpaired t-test revealed that Spargel levels are diminished in dfmr1 mutant heads compared to iso31Bw- controls (p = 0.0074). Sample number (N) per genotype = 5. Each sample contained 10 fly heads. Values represent mean ± SEM. **p ≤ 0.01. C Western analysis of Spargel expression in extracts from iso31Bw- wild type, dilp2/+ heterozygous mutant, dfmr1 homozygous mutant, and dilp2/+, dfmr1 double mutant fly heads. See Supplemental Fig. 4 for full blot. D An unpaired t-test indicated that dilp2/+, dfmr1 double mutant flies have higher Spargel expression than dfmr1 single mutants (p = 0.0088). Sample number (N) per genotype = 3. Each sample contained 10 fly heads. Values represent mean ± SEM. **p ≤ 0.01.