Table 4.
Body, organ and BALF cell data of mice exposed to towels with e-cig aerosols compared to control mice exposed to towels with no e-cig aerosols (ENDS-contaminated vs. control)
| Exposed | Control | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Characteristic | all exposed mice (n = 7) | Males (n = 5) | Females (n = 2) | 48 days (n = 3) | 75 days (n = 4) | All control mice (n = 6) | Males (n = 4) | Females (n = 2) | 48 days (n = 2) | 75 days (n = 4) | P value |
| Start weight (g) | 19.1 ± 2.0 | 20.2 ± 1.1 | 16.5 ± 0.71 | 21.0 ± 0.0 | 17.8 ± 1.5 | 22.7 ± 2.3 | 22.5 ± 3.0 | 23.0 ± 0.0 | 20.0 ± 1.4 | 24.0 ± 1.2 | 0.017 |
| End weight (g) | 21.4 ± 2.6 | 22.6 ± 2.07 | 18.5 ± 0.7 | 24.0 ± 1.0 | 19.5 ± 1.3 | 24.3 ± 1.8 | 24.8 ± 2.1 | 23.5 ± 0.7 | 23.5 ± 2.1 | 24.8 ± 1.7 | 0.043 |
| Liver (g) | 1.1 ± 0.1 | 1.2 ± 0.08 | 1.0 ± 0.01 | 1.2 ± 0.1 | 1.0 ± 0.1 | 1.1 ± 0.2 | 1.2 ± 0.2 | 0.8 ± 0.1 | 1.3 ± 0.1 | 1.0 ± 0.2 | 0.604 |
| Lung (g) | 0.4 ± 0.1 | 0.4 ± 0.06 | 0.3 ± 0.01 | 0.4 ± 0.1 | 0.4 ± 0.04 | 0.4 ± 0.1 | 0.4 ± 0.1 | 0.4 ± 0.1 | 0.3 ± 0.1 | 0.4 ± 0.1 | 0.851 |
| Brain (g) | 0.4 ± 0.04 | 0.4 ± 0.04 | 0.5 ± 0.001 | 0.4 ± 0.03 | 0.5 ± 0.03 | 0.5 ± 0.02 | 0.5 ± 0.03 | 0.4 ± 0.02 | 0.4 ± 0.03 | 0.5 ± 0.02 | 0.306 |
| Nicotine (ng/mL) | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA |
| Cotinine (ng/mL) | 6.3 ± NA | 6.3 ± NA | NA | 6.3 ± NA | NA | NA | NA | NA | NA | NA | NA |
| 3-OH Cotinine (ng/mL) | 2.5 ± NA | 2.5 ± NA | NA | 2.5 ± NA | NA | NA | NA | NA | NA | NA | NA |
| Cotinine-N-Oxide (ng/mL) | 47.8 ± NA | 47.8 ± NA | NA | 47.8 ± NA | NA | NA | NA | NA | NA | NA | NA |
| Total number of cells per ml in the BALF | 1.7E + 05 ± 1.1E + 05 | 1.9E + 05 ± 1.1E + 05 | 1.2E+05 ± 1.6E + 05 | 1.9E+05 ± 1.0E + 05 | 1.5E + 05 ± 1.4E + 05 | 5.8E + 05 ± 6.7E + 05 | 6.8E + 05 ± 8.2E + 05 | 3.6E + 05 ± 3.0E + 05 | 1.1E + 06 ± 1.2E + 06 | 3.3E + 05 ± 1.8E + 05 | 0.060 |
| Eosinophil count | 2.9E + 02 ± 7.5E + 02 | 4.0E + 02 ± 8.9E + 02 | 1.8E+01 ± 2.5E + 01 | 0 | 5.1E + 02 ± 9.9E + 02 | 4.4E + 02 ± 7.2E + 02 | 6.6E + 02 ± 8.2E + 02 | 0 | 8.4E + 02 ± 1.2E + 03 | 2.4E + 02 ± 4.9E + 02 | 0.716 |
| Lymphocyte count | 8.1E + 03 ± 7.6E + 03 | 9.5E + 03 ± 8.2E + 03 | 4.8E + 03 ± 6.5E + 03 | 6.1E + 03 ± 2.5E + 03 | 9.7E + 03 ± 1.0E + 04 | 2.5E + 04 ± 3.0E + 04 | 3.2E + 04 ± 3.5E + 04 | 1.2E + 04 ± 1.6E + 04 | 5.1E + 04 ± 4.4E + 04 | 1.2E + 04 ± 1.2E + 04 | 0.175 |
| Macrophage count | 1.5E + 05 ± 1.1E + 05 | 1.7E + 05 ± 1.0E + 05 | 1.1E + 05 ± 1.4E + 05 | 1.8E + 05 ± 1.0E + 05 | 1.4E + 05 ± 1.2E + 05 | 5.7E + 05 ± 5.7E + 05 | 6.1E + 05 ± 7.4E + 05 | 5.0E + 05 ± 4.9E + 04 | 9.6E + 05 ± 1.1E + 06 | 3.7E + 05 ± 1.4E + 05 | 0.085 |
| Neutrophil count | 6.9E + 02 ± 1.1E + 03 | 7.9E+02 ± 1.3E+03 | 4.4E+02 ± 4.8E+02 | 2.5E+02 ± 4.3E+02 | 1.0E+03 ± 1.3E+03 | 3.1E + 03 ± 3.6E + 03 | 4.7E + 03 ± 3.5E + 03 | 0 | 4.0E+03 ± 3.3E+03 | 2.7E + 03 ± 4.2E + 03 | 0.117 |
Results are presented as mean ± standard deviation (sd) for all exposed (n = 7) and all control (n = 6) mice. Under each exposure category, mean ± sd are presented by age and sex where applicable. Please note that only starting and ending mouse ages are reported in this table for readability. P values are the results of one-way analysis of variance (ANOVA) comparing control and exposed mice, where bolded and underlined P values are ≤ 0.05 and < 0.1, respectively. NA means not measured and/or not estimated.