Figure 5. Dysregulation of mycobacterial 3′,5-cyclic adenosine monophosphate (cAMP) level impacts mycobacterial survival in vivo.
(A, B) Survival of mycobacterial strains in mice (A) lung and (B) spleen after animals were given an aerosol infection with ~100 CFU/lung. Mycobacterial load represents mean CFU values with standard deviations obtained from at least five animals per strains used (**p<0.01; ***p<0.001; ****p<0.0001). (C) Histopathology of lung sections after 4 wk of infection with indicated bacterial strains. Sections were stained with hematoxylin and eosin, observed under a light microscope, and images of the pathology sections collected at ×40 magnification display granulomas (filled arrows) and alveolar space (empty arrows) (scale bar, 200 µm).
Figure 5—source data 1. CFU of indicated mycobacterial strains in mice lung (lung burden).
elife-92136-fig5-data1.xlsx (26.9KB, xlsx)
Figure 5—source data 2. CFU of indicated mycobacterial strains in mice spleen (spleen burden).
elife-92136-fig5-data2.xlsx (27.2KB, xlsx)
Figure 5—source data 3. Histopathology of lung section unlabeled.
elife-92136-fig5-data3.pptx (6.2MB, pptx)
Figure 5—source data 4. Histopathology of lung section of indicated strains.
elife-92136-fig5-data4.pptx (6.2MB, pptx)