Figure 2.

Forskolin-induced neuronal differentiation of 50B11 DRG cells. (A) Three different cultures, undifferentiated control (top) and cells undergoing differentiation (middle, bottom) were monitored up to 72 hours. Differentiation induction is mediated by the addition of forskolin, with fresh replacements at every 24 hours to maintain differentiation (bottom), compared to no replacements (middle). Cells were imaged at 0, 3, 6, 12, 24, 36, 48, 60, and 72 hours of differentiation induction. Each image represents max intensity projection (MIP) of 3D RI map. Neurite outgrowth is observed as early as 3 hours post-differentiation. Scale bar: 10 µm. Changes in (B) cell volume, (C) cell dry mass, and (D) cell body density during neuronal differentiation. (***, P ≤ 0.001; **, P ≤ 0.01; *, P ≤ 0.05)