Fig. 4.

Effects of Nef EVs on myelin in cerebellar slice cultures. Cerebellar slice cultures derived from C57BL/6J mice at postnatal day 10 were cultured for 4 days and subsequently treated with either Ctrl or Nef EVs once daily for 2 or 4 days. A Representative fluorescence images depict myelin (MBP, red) and axons (NF, green) 2 days after treatment. B Quantification of unmyelinated axon length 2 days post-treatment, measured as MBP-/NF + axon length in µm. Axons were measured on a single slice each for untreated (n = 33 axons), Ctrl EV-treated (n = 23 axons), and Nef EV-treated (n = 31 axons) groups. C Representative fluorescence images display myelin (MBP, red) 4 days after treatment. D Quantification of the percentage of MBP + immunofluorescence per field. N = 2–3 sections per treatment condition. Scale bar is 100 µm. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001