Fig. 7.

Treatment of brain cells with agents targeting Nef-mediated downmodulation of ABCA1. Mouse primary brain cells were collected at postnatal day 3 and allowed to grow for 7 days in media conducive to oligodendrocyte development. Subsequently, they were pre-treated with the LXR agonist TO-901317 (TO, 1 µM) or the inhibitor of the Nef-calnexin interaction, AMS-55 (AMS, 1 µM), for 3 days, followed by treatment with either Ctrl or Nef EVs for 48 h. A—Representative fluorescence images display oligodendrocytes (O4, green) and nuclei (DAPI, blue). B Quantification of total O4 + oligodendrocytes as a proportion of total DAPI + cells. C Quantification of the percentage of O4 immunofluorescence. Scale bar is 50 µm. N = 4. *p < 0.05; **p < 0.01; ****p < 0.0001