Table 1.
Antibodies used for immunohistochemistry
| Antibodies | Vendor | Catalog # | Clone | Dilution | Use |
|---|---|---|---|---|---|
| Mouse anti-myelin basic protein (MBP) | Biolegend | 836504 | SMI-99 | 1:500 | Detection of myelin |
| Mouse anti-APC | Millipore | MABC200 | CC1 | 1:500 | Identify mature oligodendrocytes |
| Rat anti-GFAP | Invitrogen | 13-0300 | 2.2B10 | 1:500 | Detection of astrocytes |
| Rabbit anti-Vimentin | Abcam | ab92547 | EPR3776 | 1:500 | Detection of astrocytes, glial precursors, and ependymal cells |
| Rabbit anti-Iba1 | Wako Chemicals | 019-19741 | polyclonal | 1:500 | Detection of microglia/macrophages |
| Chicken anti-Neurofilament M | Biolegend | 822701 | Poly28227 | 1:500 | Detection of axons |
| mouse anti-O4 IgM | R&D Systems | MAB1326 | 04 | 1:50 | Identify oligodendrocytes |
| Goat anti-mouse Alexa 594 | Invitrogen | A11032 | – | 1:500 | Secondary |
| Goat anti-mouse Alexa 488 | Invitrogen | A11029 | – | 1:500 | Secondary |
| Goat anti-rabbit Alexa 594 | Invitrogen | A11012 | – | 1:500 | Secondary |
| Goat anti-rabbit Alexa 488 | Invitrogen | A11008 | – | 1:500 | Secondary |
| Goat anti-rat Alexa 594 | Invitrogen | A11007 | – | 1:500 | Secondary |
| Goat anti-rat Alexa 488 | Invitrogen | A11006 | – | 1:500 | Secondary |
| Goat anti-chicken Alexa 647 | Invitrogen | A21449 | – | 1:500 | Secondary |
| Goat anti-mouse IgM Alexa 488 | Invitrogen | A21042 | – | 1:300 | Secondary |