Fig. 4.

Fibroblast contribution to the breast microenvironment. A Normal and tumor organoid growth is dependent on the microenvironment. The area was determined by DIC and normalized to the area obtained in the A/7 culture media. B Normal and cancer-associated fibroblasts (CAFs) secrete FGF-7 and AREG. Each point represents a different patient sample. C CAFs secrete higher levels of leptin, CXCL1 and CXCL5 than normal fibroblasts. Each point represents a different patient sample. D CXCL5 increases the K14 + population in organoids generated from normal breast epithelium as determined by the probability density distribution. N ≥ 2 patients with ≥ 22 organoid sections analyzed for each condition. E Heat map showing the bin distribution for each culture condition. F Representative images of organoids generated from normal breast tissue cultured in the indicated cytokine are shown. G Leptin, CXCL1 and CXCL5 do not increase proliferation in organoids generated from normal breast epithelium. See Figures S2, S3 and Tables S3 and S10