TABLE 4.
RT activities of dNTP-binding site mutants of MLV RT
| MLV RT genotype | % RT activity (mean ± SE)a | Viral titer (%)b |
|---|---|---|
| Wild type | 100 | 100 |
| Mutant | ||
| T147A | 15.4 ± 0.9 | 2.0 |
| V148D | 3.0 ± 0.9 | <0.01 |
| L149W | 1.0 ± 0.3 | <0.01 |
| D150E | 1.3 ± 0.4 | <0.01 |
| L151F | 86.7 ± 0.4 | 12 |
| K152A | 37.9 ± 15 | 71 |
| K103L | 0.9 ± 0.5 | <0.01 |
| K103R | 15.6 ± 4.5 | 4.0 |
| K103W | 0.5 ± 0.2 | <0.01 |
| R110C | 0.8 ± 0.6 | <0.01 |
| R110H | 0.9 ± 0.6 | <0.01 |
| R110I | 0.5 ± 0.1 | <0.01 |
| R110L | 0.2 ± 0.1 | <0.01 |
| R110M | 0.3 ± 0.0 | <0.01 |
| R110S | 0.3 ± 0.1 | <0.01 |
| R110T | 0.4 ± 0.1 | <0.01 |
| D153A | 6.7 ± 1.0 | 0.07 |
| D153C | 9.9 ± 2.8 | 0.07 |
| D153F | 2.4 ± 0.3 | <0.01 |
| D153Q | 8.0 ± 0.6 | 0.1 |
| D153R | 1.3 ± 0.7 | <0.01 |
| D153S | 23. ± 4.3 | 3.0 |
| A154E | 0.3 ± 0.1 | <0.01 |
| A154F | 0.2 ± 0.0 | <0.01 |
| A154L | 0.4 ± 0.0 | <0.01 |
| A154N | 0.2 ± 0.1 | <0.01 |
| A154P | 0.3 ± 0.1 | <0.01 |
| A154R | 0.4 ± 0.2 | <0.01 |
| A154S | 5.5 ± 0.9 | 1.5 |
| F155D | 0.2 ± 0.2 | <0.01 |
| F155E | 0.9 ± 0.1 | <0.01 |
| F155I | 4.9 ± 3.6 | <0.01 |
| F155M | 2.0 ± 0.4 | <0.01 |
| F155V | 5.5 ± 2.6 | <0.01 |
| F155W | 8.0 ± 3.5 | 0.04 |
| F155Y | 161.4 ± 36.9 | 31 |
| F156I | 0.8 ± 0.4 | <0.01 |
| F156L | 2.4 ± 1.3 | 0.6 |
| F156M | 8.5 ± 3.1 | 0.6 |
| F156Q | 1.1 ± 0.5 | <0.01 |
| F156V | 1.5 ± 0.3 | <0.01 |
| F156W | 11.1 ± 0.9 | 1.6 |
| F156Y | 14.8 ± 1.2 | 3.0 |
| C157A | 56.1 ± 6.0 | 110 |
| R159A | 30.6 ± 6.5 | 51 |
| H161A | 43.6 ± 19.1 | 45 |
| Q190E | 1.0 ± 0.4 | <0.01 |
| Q190H | 0.3 ± 0.1 | <0.01 |
| Q190M | 51.6 ± 2.2 | 60 |
| Q190N | 0.5 ± 0.1 | <0.01 |
Average RT activities determined using virion-associated RT. The activities shown are relative to the wild-type MLV RT (set to 100%) using the poly(rA)-oligo(dT) template-primer complex. The results represent averages of two to three experiments. RT activities of all mutants seem to be statistically different from that of the wild-type MLV RT (P < 0.002), except for those of the L151F, K152A, F155Y, C157A, and H161A mutants (P > 0.05).
Viral titers of mutants as a percentage of the wild-type titer (set at 100%). Viral titers represent the ratio of the virus titer generated from mutant MLV RT obtained in each experiment divided by the virus titer generated from the wild-type MLV RT in parallel experiments and multiplied by 100.