Figure 1.

Structure of bacteriophage (phage)-derived particle (PDP)

(A) Schematic representation of the RGD4C.PDP-sTRAIL particles. PDP comprises three alterations: (1) the pIII minor coat proteins display five copies of the double cyclic RGD4C ligand, (2) the recombinant pVIII major coat proteins express the EEP H5WYG, and (3) the PDP capsid packages a mammalian transgene cassette for sTRAIL expression, flanked by AAV2 ITRs. The f1 ori (phage origin of replication), which enables single-stranded DNA replication and packaging into the phage capsid, is the only remaining phage genetic sequence. (B) Electron microscopic images of the PDP particles, targeted RGD4C.PDP-sTRAIL and non-targeted PDP-sTRAIL are shown. Scale bar, 200 nm.