Figure 5.

In vitro cell death of CS mediated by a PDP DNA construct carrying a recombinant sTRAIL DNA (PDP-sTRAIL)

Human CS SW1353 cells at 60% confluency on 96-well plates were transfected with 6 μg DNA of PDP-sTRAIL plasmid using the FuGENE6 reagent. (A) Quantification of the secreted sTRAIL in the culture medium of cells by ELISA. (B) Brightfield imaging and crystal violet staining of cells showing CS cell morphology and confluence on day 2 after transfection. Scale bar, 100 μm. (C) Evaluation of cell death using a Cell Titer-Glo cell viability assay kit. (D) Analysis of caspase 3/7 activity using a Caspase-Glo 3/7 Assay System. Results are shown as mean ± SEM of triplicate wells of one representative experiment and compared with the MOCK transfection control group. All experiments were repeated twice. ∗∗∗p < 0.001.