Fig. 6.

LDHB suppresses HCC progression in immune-competent mice but not in immune-deficient mice. A LDHB expression in Hep3B/LDHB and corresponding control cells was examined by Western blotting. B Cell proliferation assay was conducted with the Hep3B cells tested in A. C Cell colony formation assay was conducted with the Hep3B cells tested in A. D LDHB expression in Hepa1-6/LDHB and corresponding control cells was examined by Western blotting. E Cell proliferation assay was conducted with the HepA1-6 cells tested in D. F Cell colony formation assay was conducted with the Hepa1-6 cells tested in D. G Hepa1-6 cells tested in D were inoculated into C57BL/6 mice (n = 5). Homograft tumors at the endpoint were collected and shown. H The weight of tumors at the endpoint in G. I The growth curve of the tumors in G. J Hepa1-6 cells tested in D were inoculated into nude mice (n = 6). Xenograft tumors at the endpoint were collected and shown. K The weight of tumors at the endpoint in J. L The growth curve of the tumors in J. M IHC staining of LDHB expression and immunofluorescent staining of CD8⁺ T cells were performed on paraffin­embedded tumor tissues. Scale bar, 100 μm for IHC; 20 μm for immunofluorescence