Skip to main content
. 2024 May 13;10:227. doi: 10.1038/s41420-024-01984-7

Fig. 12. Triton X-100 or IFN-γ and TNF-α induces PANoptosis in CT26 and CMT93 MLH1 KO or CT26 and CMT93 Vector Control.

Fig. 12

CT26 and CMT93 MLH1 KO, CT26, and CMT93 Vector Control were treated by Triton X-100 at 0.001% v/v for 0 h, 6 h, or 12 h (a–d); CT26 and CMT93 Vector Control, CT26 and CMT93 MLH1 KO or CT26 MLH1 KO + Mlh1 were co-treated with IFN-γ + TNF-α, IFN-γ + TNF-α + Emricasan, IFN-γ + TNF-α + Z-IETD-FMK or IFN-γ + TNF-α + Nec-1 for 36 h (e, g); or treated with 0.001% v/v Triton X-100 for 12 h after treatment with Emricasan alone, Z-IETD-FMK alone or Nec-1 alone for 24 h (f, h). All cells were stained by PI and time-lapse confocal images of PI + DIC. Two-tailed unpaired Student’s t-test was used to determine significance (*P < 0.05; **P < 0.01; ***P < 0.001; NS, not significant). Scale bars, 50 um. Data are representative of at least three independent experiments.