Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2024 May 13;10:227. doi: 10.1038/s41420-024-01984-7

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 13 — a–m, o–t 2 × 10⁵ CT26 (Ctrl), CT26 KO1, CT26KO2, or CT26 KO2 + Mlh1 were injected 180 days after genome editing into syngeneic BALB/c mice at the indicated time points (n = 6) (i); Multiplex immunohistochemistry of Perforin, Granzyme B, Cleaved-CASP3, IFN-γ, TNF-α, and DAPI (a, c, e, g, j, l); N-GSDMD, N-GSDME, Cleaved-CASP3, Cleaved-CASP8, p-MLKL, and DAPI (b, d, f, h, k, m); NK.1.1, F4/80, CD8, Perforin, Granzyme B, and DAPI (o, q, s); NK.1.1, F4/80, CD8, IFN-γ, TNF-α, and DAPI (p, r, t) in the CT26 (Ctrl), CT26 KO1, CT26KO2 or CT26 KO2 + Mlh1 tumor samples from i. The images are representative sections from one mouse. The staining was performed on six independent mice. n 10⁶ CT26 clones were injected into syngeneic BALB/c mice (n = 7). Counts of NK1.1, F4/80, CD8+, Perforin, Granzyme B, IFN-γ, and TNF-α per high-power field from representative fields from each experimental arm (CT26 (Ctrl) group (n = 6), CT26KO2 group (n = 5) and CT26 KO2 + Mlh1 group (n = 4)). Experiments were repeated at least two times. Data are presented as mean ± SEM. Statistical analysis was performed using one-way ANOVA (i, n) and two-tailed unpaired Student’s t-test (u) (*P < 0.05; **P < 0.01; ***P < 0.001; NS, not significant). Ctrl, control. Scale bars, 20 μm. v, Schematic of dsDNA fragments driven hyperactivation of pyroptosis and IFN-γ and TNF-α-induced cell death in dMMR cancer cells.

Fig. 13 — a–m, o–t 2 × 10⁵ CT26 (Ctrl), CT26 KO1, CT26KO2, or CT26 KO2 + Mlh1 were injected 180 days after genome editing into syngeneic BALB/c mice at the indicated time points (n = 6) (i); Multiplex immunohistochemistry of Perforin, Granzyme B, Cleaved-CASP3, IFN-γ, TNF-α, and DAPI (a, c, e, g, j, l); N-GSDMD, N-GSDME, Cleaved-CASP3, Cleaved-CASP8, p-MLKL, and DAPI (b, d, f, h, k, m); NK.1.1, F4/80, CD8, Perforin, Granzyme B, and DAPI (o, q, s); NK.1.1, F4/80, CD8, IFN-γ, TNF-α, and DAPI (p, r, t) in the CT26 (Ctrl), CT26 KO1, CT26KO2 or CT26 KO2 + Mlh1 tumor samples from i. The images are representative sections from one mouse. The staining was performed on six independent mice. n 10⁶ CT26 clones were injected into syngeneic BALB/c mice (n = 7). Counts of NK1.1, F4/80, CD8+, Perforin, Granzyme B, IFN-γ, and TNF-α per high-power field from representative fields from each experimental arm (CT26 (Ctrl) group (n = 6), CT26KO2 group (n = 5) and CT26 KO2 + Mlh1 group (n = 4)). Experiments were repeated at least two times. Data are presented as mean ± SEM. Statistical analysis was performed using one-way ANOVA (i, n) and two-tailed unpaired Student’s t-test (u) (*P < 0.05; **P < 0.01; ***P < 0.001; NS, not significant). Ctrl, control. Scale bars, 20 μm. v, Schematic of dsDNA fragments driven hyperactivation of pyroptosis and IFN-γ and TNF-α-induced cell death in dMMR cancer cells.