Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2024 May 13;10:227. doi: 10.1038/s41420-024-01984-7

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 6 — Immunoblot analysis of pro-(P53), activated (P30), oligomerized GSDMD, and pro-(P53), activated (P34), oligomerized GSDME (a); tMLKL, phosphorylated MLKL (pMLKL) and oligomerized pMLKL (b) induced by PBS or IFN-γ and TNF-α in DLD1 or HCT116; the active NT-GSDMD, NT-GSDME, p-MLKL could form oligomers, 100kD, 130KD, 180KD, and bigger oligomers. The active NT-GSDMD, NT-GSDME mainly in the cytosolic fraction and membrane fraction, little in the nuclear fraction; the active p-MLKL is mainly in the nuclear fraction, little in the cytosolic fraction and membrane fraction. ATP, GAPDH, and Lamin B1 were used as membrane, cytosolic, and nuclear internal control respectively. Data are representative of at least three independent experiments.