FIG. 4.

Efficient replication of mAd genome in C12 cells (6). HeLa and C12 cells were infected with CsCl₂-purified Ad/AAV recombinant virus at an MOI of 10 PFU/cell with (+) or without (−) wild-type Ad5 helper virus. Replicated DNA (15) was isolated 24 h after infection and analyzed by Southern blot using Ad5 (nt 1 to 355) (A) and EGFP/Neo (B) as DNA probes. The majority of the replicated DNA in HeLa cells was full-length Ad/AAV DNA (F). In C12 cells most of the replicated DNA represents monomer (M) and dimer (D) forms of mAd genome. The arrow indicates a submonomer band that was found only in C12 cells. Markers are indicated as kilobase pairs on the left.