FIG. 7.

Model for mAd formation. (A) Parental Ad/AAV hybrid virus. Ad replicates using a strand displacement mechanism. During that process a single strand is released. (B) The AAV TR-DD in that strand forms an AAV secondary structure. After Rep-mediated cleavage and DNA extension (B and C), the Ad second strand is synthesized (D), leading to the production of the dimer genome (E). The monomeric form of mAd (F) could be generated by homologous recombination between two AAV D direct repeats or through the mechanism proposed by Steinwaerder et al. (29).