FIG. 4.

Capture of HCV HMAbs and E2 onto CD81-coated plates. Proteins derived from 15 μl of extract derived from BSC-1 cells infected with vaccinia virus Q1b and VWA were combined with the indicated concentrations (x axis) of antibody in a total volume of 100 μl of BLOTTO. The antibody-protein mixture was then added to microtiter plate wells coated with 100 ng of a GST–CD81-LEL fusion protein or nonrecombinant GST and incubated overnight at 4°C. Wells were washed, and bound antibody was detected as described in Materials and Methods. Signals obtained for antibody captured by CD81-coated wells were subtracted from signals obtained from GST-coated wells and averaged. R04 is an isotype matched HMAb that recognizes a CMV protein (14). Values are the average of duplicate wells. Error bars indicate 1 standard deviation from the mean.