FIG. 6.
Reactivity of HCV HMAbs to preformed CD81-E2 complexes. Proteins derived from 3 × 105 BSC-1 cells infected with vaccinia virus VWA with Q1a (■, □), Q1b (▴, ▵), Q2a (▾, ▿), and Q2b (⧫, ◊) were added to microtiter plate wells coated with human CD81-LEL (closed symbols and solid lines) or murine CD81-LEL (open symbols and dotted lines) and incubated overnight at 4°C. Wells were washed and then incubated with increasing amounts (x axis) of the indicated HCV HMAb. Bound antibody was detected as described in Materials and Methods. Values for murine CD81 are derived from a single determination. Values for human CD81 are the means of duplicate wells. Error bars indicate 1 standard deviation from the mean.