Figure 1.

DUPA-miR-34a conjugation and binding to prostate cancer cells

(A) Effect of transfecting various tumor suppressive miRNAs on proliferation of LNCaP cells, measured by SRB assay. Experiment repeated twice with five technical replicates in each experiment. One representative biological replicate is shown, one-way ANOVA. ∗∗∗∗p < 0.0001, error bars are mean ± SD. (B) Schematic of DUPA-miR-34a synthesis. (C) Gel red-stained polyacrylamide gel indicating successful conjugation of DUPA-miR-34a based on the shift in mobility. (D) Flow cytometry histograms of miR-34a-Atto.647N (with or without DUPA conjugation) treated cells in the presence or absence of the PSMA inhibitor, PMPA. (E and F) Flow cytometry analysis of DUPA binding to PSMA positive cancer cells in a dose-dependent (E) and time-dependent (F) manner using DUPA-AF488.